《植物生理学报》 2015, 51(1): 105-111

4-BDE对毛白杨组培苗的显微结构和一些生理指标的影响

才满^1,2, 李燕玲¹, 杜克久^1,2,*

河北农业大学¹林学院, ²河北省林木种子资源与森林保护重点实验室, 河北保定071000

通信作者：杜克久；E-mail: dukejiu@126.com；Tel: 13833072220

摘　要：

本文研究了4-BDE处理对毛白杨组培苗的形态结构以及生理生化特性的影响。结果表明, 在低浓度4-BDE处理下, 组培苗叶片颜色变浅, 随4-BDE处理浓度增加, 新叶出现失绿白化现象。与对照苗相比, 4-BDE处理的显微结构发生了明显改变, 根的表皮细胞损伤现象严重, 细胞形态变小; 叶肉组织遭到破坏, 海绵组织破坏程度高于栅栏组织; 但茎的结构与正常苗无显著差异。30 mg•L^-1 4-BDE处理23 d的白化叶片比叶重显著低于对照, 其他各处理均无显著差异。叶片中叶绿素含量随4-BDE浓度的升高而下降, 与4-BDE存在显著的浓度抑制关系。30 mg•L^-1 4-BDE处理23 d的毛白杨丙二醛含量则显著低于处理47和58 d的。随着4-BDE处理时间的增加, 过氧化物酶活性显著下降, 而脯氨酸含量呈先降低再升高的趋势。以上结果初步显示, 毛白杨组培苗对低于30 mg•L^-1 4-BDE的污染具有一定的修复能力。

关键词：4-BDE; 毛白杨组培苗; 形态结构; 生理指标

收稿：2014-09-30   修定：2014-12-25

资助：“十二五”农村领域国家科技计划课题(2012AA101403)和环境化学与生态毒理学国家重点实验室开放基金课题(KF2009-03)。

Effects of 4-BDE on Microstructure and Some Physiological Indexes in Populus tomentosa Seedlings

CAI Man^1,2, LI Yan-Ling¹, DU Ke-Jiu^1,2,*

¹College of Forestry, ²Hebei Provincial Key Laboratories for Germplasm Resources of Forest Trees and Forest Protection, Hebei Agriculture University, Baoding, Hebei 071000, China

Corresponding author: DU Ke-Jiu; E-mail: dukejiu@126.com; Tel: 13833072220

Abstract:

This research was performed to investigate the effects of 4-BDE on morphological structure and physiological-biochemical characteristics on tissue culture plantlet of Populus tomentosa. The results indicated that leaf color became lighter with low concentration of 4-BDE, the new leaves appeared albinism with 4-BDE concentration increased. Comparing with control, seedlings showed significantly abnormal microstructure under 4-BDE treatment. The root epidermis had serious damaged and the size of cells became smaller. Mesophyll tissue was destroyed, the damaged extent of spongy mesophyll tissue was higher than that of palisade. But the microstructure of stem showed no differences. Under 30 mg•L^-1 4-BDE treatment for 23 days, the leaf mass per area of albino leaf was significantly lower than the control, other treatment had no significant difference. With 4-BDE concentration increased, chlorophyll content of leaves decreased, showing definite dose-response relationships. MDA content with 30 mg•L^-1 4-BDE for 23 days was significantly lower than 47 days and 58 days. With 4-BDE treatment time increased, peroxidase activity displayed the decreasing trend, while proline content decreased at frist and then increased. The above results showed that P. tomentosa seedlings had certain repairing ability in the range of less than 30 mg•L^-1 4-BDE.

Key words: 4-BDE; Populus tomentosa seedling; microstructure; physiological indexes